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61.
Marrubium vulgare (Lamiaceae) is a plant traditionally used for the treatment of diabetes in Algeria. Compositional analysis of the aqueous infusion revealed the presence of fifteen metabolites, all belonging to the class of polyphenols. Particularly, seven flavonoids have been detected, together with 5-caffeoylquinic (chlorogenic) acid in small amounts; the extract is dominated by the presence of a series of complex molecules, characterized as verbascoside (acteoside) derivatives. Concerning the anti-diabetic effectiveness a series of in vivo experiments were carried out on albinos Wistar rats. Diabetes was induced in the animals by intra-peritoneal injection of alloxane; they were treated twice a day with aqueous extract from aerial part infusion (100, 200 and 300 mg/kg body weight) and glibenclamide (5mg/kg body weight) for 15 days. Oral administration of 200 and 300 mg/kg body weight of aqueous extract the Marrubium vulgare induced an significant effect antidiabetic and antihyperlipidemic (dose-dependent effect). A decrease in blood glucose by 50% for the dose 100 mg/kg and more than 60% for doses 200 and 300 mg/kg, as well as a significant lowering of total lipids, triglycerides, and total cholesterol levels in treated animals, compared with diabetic controls group (p<0.001), have been observed. Glibenclamide was used as reference and showed similar effects. 相似文献
62.
Marco Quartuccio Gabriele Marino Giuseppe Garufi Santo Cristarella Antonina Zangh�� 《Journal of veterinary science (Suw?n-si, Korea)》2012,13(2):207-209
The association of cryptorchidism, functional Sertoli cell tumors, and spermatic cord torsion has been rarely reported in the literature. Two dogs were admitted for bilateral skin alopecia and weight loss. Both animals were cryptorchid and displayed a pendulous preputial sheath, prostate hypertrophy, and increased levels of circulating oestrogen. Transabdominal palpation and ultrasonography revealed the presence of neoplastic retained gonads. During surgery, spermatic cord torsion was also detected in the enlarged neoplastic testes of both dogs. Histologic examination confirmed the presence of Sertoli cell tumors that were primarily responsible for the feminizing syndrome. Complete remission of all symptoms occurred within 3 months after orchiectomy. 相似文献
63.
Youssef Rouphael Mariateresa Cardarelli Elena Di Mattia Monica Tullio Elvira Rea Giuseppe Colla 《Biology and Fertility of Soils》2010,46(5):499-509
The aim of the present study was to determine whether arbuscular mycorrhizal (AM) inoculation with a biofertilizer containing
clays as granular carriers, leek root pieces and Glomus intraradices spores could improve alkalinity tolerance of two cucumber genotypes, and to study the changes induced by AM at agronomical
and physiological level. A greenhouse experiment was carried out to determine yield, growth, fruit quality, net photosynthesis
(ACO2), electrolyte leakage, and mineral composition of two cucumber (Cucumis sativus L.) genotypes (hybrid “Ekron” or open-pollinated variety “Marketmore”) with inoculated and noninoculated arbuscular mycorrhizal
biofertilizer. Plants were supplied with nutrient solutions at two pH values (6.0 or 8.1). The high pH nutrient solution had
the same basic composition with an additional 10 mM NaHCO3 and 0.5 g l−1 CaCO3. The percentage root colonization was higher in “Marketmore” (21.8%) than “Ekron” (12.7%). Total and marketable yield and
total biomass were significantly higher by 189%, 213%, and 77%, respectively, with Ekron in comparison to those recorded with
Marketmore. The highest crop performance with Ekron in comparison to Marketmore was due to the improved nutritional status
(higher N, P, K, Ca, Mg, Fe, Mn, and B), higher leaf area, and net photosynthesis. Increasing the concentration of NaHCO3 from 0 to 10 mM in the nutrient solution significantly decreased yield, plant growth, ACO2, N, P, Fe, Cu, Zn, Mn, and B concentration in leaf tissue, whereas the electrolyte leakage increased. The inoculated plants
under alkaline conditions had higher total, marketable yield, and total biomass than noninoculated plant. Mycorrhizal cucumber
plants grown under alkaline conditions had a higher macronutrient concentration in leaf tissue compared to noninoculated plants.
The highest yield and biomass production in inoculated plants seems to be related to the capacity of maintaining higher net
ACO2 and to a better nutritional status (high P, K, Mg, Fe, Zn, and Mn and low Na accumulation) in response to bicarbonate stress
with respect to −AM plants. 相似文献
64.
Giuseppe Piccione Claudia Giannetto Simona Marafioti Michele Panzera Anna Assenza Francesco Fazio 《Journal of Veterinary Behavior: Clinical Applications and Research》2013,8(1):40-45
The activity rhythm can be entrained by nonphotic cues, especially food availability. Daily feeding schedules can act as “Zeitgeber” to synchronize the circadian system. In this study, the authors evaluated the influence of different feeding schedules on the daily rhythm of total locomotor activity in ponies. Six clinically healthy Shetland ponies were kept under natural 12/12-h light/dark cycle and monitored during 4 different feeding schedules: fed twice (at 8 am and 8 pm), fasted, fed at 8 pm, fed at 8 am, and fed twice a day again. Water was available ad libitum. To record total activity, we equipped the animals with actigraphy-based data loggers (Actiwatch-Mini). Two-way analysis of variance revealed a significant main effect of the time of day and no effect of feeding schedules on the amount of total locomotor activity. Locomotor activity showed daily rhythmicity in all experimental conditions, with diurnal acrophase. The amplitude of the rhythms and their robustness statistically changed across all experimental conditions. Our findings support the theory that when given essentially free choice hay, the time of feeding did not entrain activity, and suggest that feeding behavior can influence the distribution of locomotor activity during a 24-hour period in ponies, with changes in the amplitude and robustness of the rhythm. 相似文献
65.
Maria Foti Cristina Giacopello Vittorio Fisichella Giuseppe Latella 《Journal of Exotic Pet Medicine》2013,22(3):270-274
Pseudomonas aeruginosa represents an opportunistic pathogen for animals and humans that is often associated with high disease morbidity and, at times, mortality. Captive reptiles have been shown to be reservoirs of P. aeruginosa strains that can be sources of exposure to humans that come in contact with these animals. In this study, the prevalence of P. aeruginosa among subclinical captive reptile species and the antimicrobial sensitivity of bacterial isolates were investigated. Sixty-five oral swabs were collected from captive reptiles belonging to 15 different species in which no overt signs of disease were evident. From this group of animals, 46 (70.8%) isolates were identified as P. aeruginosa. All of the P. aeruginosa strains were shown to have a wide range of antibiotic resistance. At present, there is a paucity of data regarding the prevalence of P. aeruginosa in various reptile species; therefore, continued scientific investigations are indicated to determine the significance of P. aeruginosa infection as it relates to captive reptile species. 相似文献
66.
Domenico Vecchio Rossella Di Palo Esterina De Carlo Luigi Esposito Giorgio Antonio Presicce Alessandra Martucciello Emilio Chiosi Pasquale Rossi Gianluca Neglia Giuseppe Campanile 《Tropical animal health and production》2013,45(8):1697-1702
Growth, weight at birth and daily weight gain (DWG) on 12 water buffalo calves, starting from 6 days of age until completion of weaning, was investigated in this study. Different feeding regimens were given to two groups of animals with regard to daily milk replacer: (1) group 1 (G1) received a double concentration in single administration; whereas (2) group 2 (G2) received the same amount of milk replacer split twice daily. Blood samples were collected from each calf on days 6, 30, 60 and 90 to evaluate acute phase proteins (haptoglobin), bactericide activity, lysozime, total protein content and biochemical parameters. No differences were observed between the two groups in terms of dry matter intake, feed efficiency and live body weight at the end of the study. Interestingly, a significantly (P?<?0.05) reduced DWG was observed earlier in G1 (day 45) than in G2 (day 60). Gastrointestinal disorders were not recorded throughout the experimental period, and no significant differences were recorded between the two groups for all considered parameters. This study confirms the possibility of utilising one daily administration of milk replacer in water buffalo calf during weaning. This new approach facilitates calves management, without interfering with calves growing performances. 相似文献
67.
Diana Binanti Giovanni Stancari Eleonora Fantinato Enrica Zucca Davide Danilo Zani Giuseppe Sironi Francesco Ferrucci Pietro Riccaboni 《Journal of Equine Veterinary Science》2013
A case of pulmonary carcinoma in a 23-year-old Quarterhorse mare is described. On physical examination, depression, poor body condition, tachypnea, bilateral serosanguineous nasal discharge and a wide area of reduced breath sounds in association with the right hemithorax were detected. Laboratory evaluation showed leukocytosis with neutrophilia and lymphopenia, hyperfibrinogenemia, hyperprotidemia, hypoalbuminemia, increased beta 2 and gamma globulin fractions, hypoxemia, and normocapnia. Radiography and thoracic ultrasonography revealed a large rounded mass extending from the 9th to the 14th right intercostal space, where neither bronchial nor vascular structures were detectable. Endoscopy showed a large amount of serosanguineous fluid within the tracheal lumen and a mass of reddish soft tissue completely obliterating the right caudal lobar bronchus. Histopathology of the endobronchial mass and of the transthoracic ultrasound-guided biopsy samples was consistent with a primary pulmonary epithelial tumor. Due to worsening of the clinical condition, the mare was euthanized. Postmortem examination confirmed the presence of a large 30-cm mass located in the right caudal pulmonary lobe, characterized by epithelial cells arranged in papillary projections and alveolar structures, findings consistent with bronchioloalveolar carcinoma. 相似文献
68.
Daniela Alberghina Stefania Casella Claudia Giannetto Simona Marafioti Giuseppe Piccione 《Canadian journal of veterinary research》2013,77(4):293-296
Serum protein electrophoresis (SPE) is a technique that could be considered one of the most useful diagnostic aids available to the clinician. The effect of storage time and temperature on the total proteins and electrophoretic fractions (albumin, α1-, α2-, β1-, β2-, and γ-globulins) was assessed in 24 healthy horses. All samples, collected by jugular vein puncture, were centrifuged and divided into 4 aliquots. The 1st aliquot was analyzed within 3 h from collection (time 0), the 2nd was refrigerated at +4°C for 24 h, the 3rd was refrigerated at +4°C for 48 h, and the last was frozen at −20°C for 48 h. One-way repeated-measures analysis of variance (ANOVA) showed a significant effect (P < 0.05) of the different storage conditions on the concentrations of all the parameters studied and significant variations in the percentages of albumin, α1-globulins, α2-globulins, and γ-globulins. Compared with time 0 the total protein concentration increased significantly after 48 h at −20°C, the albumin percentage decreased after 48 h at −20°C, the α1-globulin percentage increased after 24 h at +4°C, the α2-globulin percentage increased after 48 h at +4°C and at −20°C, and the γ-globulin percentage increased after 48 h at −20°C. The results should help veterinary practitioners handle and store equine serum samples appropriately. Further investigations at different storage times and temperatures could be useful. 相似文献
69.
Roberta Teta Elena Irollo Gerardo Della Sala Giuseppe Pirozzi Alfonso Mangoni Valeria Costantino 《Marine drugs》2013,11(11):4451-4463
An in-depth study of the secondary metabolites contained in the Caribbean sponge Smenospongia aurea led to the isolation of smenamide A (1) and B (2), hybrid peptide/polyketide compounds containing a dolapyrrolidinone unit. Their structures were elucidated using high-resolution ESI-MS/MS and homo- and heteronuclear 2D NMR experiments. Structures of smenamides suggested that they are products of the cyanobacterial metabolism, and 16S rRNA metagenomic analysis detected Synechococcus spongiarum as the only cyanobacterium present in S. aurea. Smenamides showed potent cytotoxic activity at nanomolar levels on lung cancer Calu-1 cells, which for compound 1 is exerted through a clear pro-apoptotic mechanism. This makes smenamides promising leads for antitumor drug design. 相似文献
70.
Vincent Niderkorn Giuseppe Copani Ccile Martin Gaëlle Maxin Anglique Torrent Frdric Anglard Yvanne Rochette Ccile Ginane 《Grass and Forage Science》2019,74(4):626-635
The aim of this work was to investigate the effects of feeding sheep with silage mixtures containing bioactive legumes on intake and digestive parameters. The bioactive legumes used were sainfoin (SF, Onobrychis viciifolia) and red clover (RC, Trifolium pratense), which contain condensed tannins (CT) and polyphenol oxidase respectively. Five treatments were assigned to two groups of sheep according to a replicated 5 × 5 Latin square design. The five types of silages tested were, on a dry matter (DM) basis: pure timothy grass silage (Phleum pratense, control, T), three binary mixtures of T‐SF, T‐RC and RC‐SF (500 g/kg each) and a ternary mixture of T‐RC‐SF (500, 250 and 250 g/kg respectively). The daily voluntary DM intake of silage mixtures containing both SF and RC was greater than for pure T silage, while the presence of SF resulted in lower organic matter digestibility compared to pure T. The rumen disappearance rate measured in situ increased linearly with the presence of SF and RC in silage. The nitrogen (N) digestibility was greater for pure T and T‐RC than for T‐SF, and the amount of N retained daily by the animals was greater for RC‐containing silages than for T and T‐SF. The methane (CH4) yield was greater for pure T than for the silage mixtures containing SF. We conclude that the presence of RC in silage could boost performances through intake and N retention, while SF‐based mixtures appear to have reduced negative environmental impacts through the reduction of CH4 emissions. 相似文献